evaluation of a rapid fluorescent staining method for

Submit Demands Online

2009/4/1Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens Cindy Hendry Kim Dionne Annie Hedgepeth Karen Carroll Nicole Parrish Journal of Clinical Microbiology Apr 2009 47 (4) 1206-1208 DOI: 10 1128/JCM 02097-08 2007/10/15A bacterial staining method using fluorescent dye is a popular tool although the weakness of fluorescence intensity and its fading over time constitute notable drawbacks In the process of esterase-active bacteria staining with carboxyfluorescein diacetate (CFDA) we have reported glutaraldehyde (GTA) affected the discriminative recognition of bacteria due to prevention of

Modified Field's staining—a rapid stain for Trichomonas

2010/10/12 4 Gram staining method of T vaginalisSmears of sediment containing T vaginalis were dried in room temperature for 15 to 20 min and stained using Gram stain (Stefanski et al 2010) following a standardized 4-step staining protocol with Hucker's modification and Becton Dickinson (Becton Dickinson and Company Franklin Lakes NJ) Gram stain kits

Evaluation of a direct fluorescent antibody staining method for rapid identification of members of the bacteroides fragilis group DeGirolami PC Mepani CP A direct fluorescent antibody test kit (Fluorotec-F Pfizer Inc New York New York) designed for rapid

Aims: To apply fluorescent staining method for fast assessment of microbial quality of herbal medicines Methods and Results: The number of total bacteria and esterase‐active bacteria on powdered traditional Chinese medicines were enumerated by fluorescent staining method using 6‐carboxyfluorescein diacetate (6CFDA) and 4′ 6‐diamidino‐2‐phenylindole (DAPI) and they were

2016/10/12With some adjustments of staining time and / or rhodamine concentration this method should also be applicable to other plant species Acknowledgments We thank the ZMBP facilities for support Abhroop Garg for his help with confocal microscopy and

Evaluation of Different Staining Methods for the Detection of Acid Fast Bacilli in Sputum Samples as the method is highly specific rapid and cheapest method used for detection of AFB in sputum The only disadvantage of this method is low sensitivityto 80%

Field Evaluation of a Simple Fluorescence Method for

2012/5/9Field Evaluation of a Simple Fluorescence Method for Detection of Viable Mycobacterium tuberculosis in Sputum Specimens during Treatment Follow-Up Birgit Schramm a Cathy Hewison b Laurence Bonte b Warren Jones c * Olivier Camlique d Ronnatrai Ruangweerayut e Witaya Swaddiwudhipong e and Maryline Bonnet f

Fazii P Ciancaglini E Riario Sforza G Differential fluorescent staining method for detection of bacteria in blood cultures cerebrospinal fluid and other clinical specimens Eur J Clin Microbiol Infect Dis 2002 21(5):373–8 CAS Article Google Scholar

Adiga Deepa and Hippargi Surekha B and Rao Gururaja K and Saha Debarshi and Yelikar Bheemrao Ramling and Karigoudar Mahesh (2016) Evaluation of Fluorescent Staining for Diagnosis of Leprosy and its Impact on Grading of the Disease: Comparison with Conventional Staining

Evaluation of a rapid fluorescent staining method for detection of mycobacteria in clinical specimens Hendry C(1) Dionne K Hedgepeth A Carroll K Parrish N Author information: (1)The Johns Hopkins Medical Institutions Baltimore Maryland 21287 USA

Pharmaceuticals and Medical Devices Agency 9 This chapter provides rapid methods using fluorescence staining for quantitative estimation of viable microorganisms Fluorescence staining method (ex CFDA (carboxyfluorescein diacetate)-DAPI (4' 6-diamidino-2

Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens Cindy Hendry Kim Dionne Annie Hedgepeth Karen Carroll and Nicole Parrish* The Johns Hopkins Medical Institutions Baltimore Maryland Received 31

Evaluation of a direct fluorescent antibody staining method for rapid identification of members of the bacteroides fragilis group DeGirolami PC Mepani CP A direct fluorescent antibody test kit (Fluorotec-F Pfizer Inc New York New York) designed for rapid

Previous studies investigating electrotonic coupling in mammalian central nervous system have used the fluorescent marker Lucifer Yellow as an indicator of the presence of intercellular junctions between neurons Fingerprint Dive into the research topics of 'Intracellular fluorescent staining with carboxyfluorescein: a rapid and reliable method for quantifying dye-coupling in mammalian central

Evaluation of acridine Orange staining for rapid detection

Each sputum sample was stained using three different staining techniques viz P-AO fluorescent staining RA fluorescent staining at 37 C and ZN stain The Acridine Orange (AO) reagent was prepared by dissolving 5 g of phenol crystals in a solution containing 50 ml of deionized water 25 ml of glycerol (Sigma Mumbai India) and 25 ml of 95% ethanol

The pork tonsils are an important carrier of Salmonella which could be involved in the contamination of pork products during the slaughter process This paper reports a 23S rRNA fluorescent in situ hybridization (FISH) method used as a rapid screening tool for Salmonella detection in tonsils of slaughtered pigs and its comparison with the conventional culture method

Adiga Deepa and Hippargi Surekha B and Rao Gururaja K and Saha Debarshi and Yelikar Bheemrao Ramling and Karigoudar Mahesh (2016) Evaluation of Fluorescent Staining for Diagnosis of Leprosy and its Impact on Grading of the Disease: Comparison with Conventional Staining

Adiga Deepa and Hippargi Surekha B and Rao Gururaja K and Saha Debarshi and Yelikar Bheemrao Ramling and Karigoudar Mahesh (2016) Evaluation of Fluorescent Staining for Diagnosis of Leprosy and its Impact on Grading of the Disease: Comparison with Conventional Staining

Abstract An evaluation of the newly developed Clinical Sciences Inc Salmonellae Fluoro-Kit which attempts to standardize the various aspects of the fluorescent-antibody (FA) procedure was performed with 120 naturally contaminated human food animal feed and

This FA method combines a rapid cultural phase and a serological phase that allow for propagation of salmonellae in a minimum time employing the industrial 8-hr work day as a guide Two hundred fifty naturally contaminated human food and animal feed samples representing 647 trials were tested by the FA method